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Quick Details

  • Type: Pathological Analysis Equipments
  • Brand Name: Green Spring
  • Place of Origin: Guangdong, China (Mainland)
  • Model Number: LSY-30036
  • Certificate: ISO9001&ISO14001&OHSMS18001
  • MOQ: 1 kit
  • Specifications: 96 wells/kit or 96*2 wells/kit
  • Sample performance: Serum
  • Shelf life: 12 months

Packaging & Delivery

Packaging Details By foam box with ice to maintain cool storage
Packing kit size: 16.5*11.5*10cm
96 wells/kit or 96*2 wells/kit
Delivery Time In 3 days after payment

Brucellosis antibody ELISA kit for bovine, sheep, pig and dog

 Catalog No. LSY-30036



Product Description
  1.  Introduction 

The Brucellosis antibody ELISA kit is used to test Brucellosis antibody in serum of bovine, sheep, pig and dog etc.

This kit use competitive ELISA method, Brucellosis antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Brucellosis specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Brucellosis virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.

2. Reagents and contents








Brucellosis-Ag Coated plates

96 wells


Stop solution

6 ml


Enzyme Conjugate

6 ml


Negative  control

150 ul


10X Concentrated washing buffer  

100 ml


Positive control

50 ul



11 ml


Adhesive Foil

2 pieces


Sample diluent

20 ml


Instruction sheet

1 piece

3. Material required not provided

1) Micropipette: 10ul-100ul, 100ul-1000ul.

2) Disposable pipette tips.

3) Graduate: 500ml.

4) Microplate Reader: 96 wells.

5) Distilled water or deionized water.

6) Microplate Washer

4. Sample preparation

Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis

5. Washing buffer preparation

Return 10X Concentrated washing buffer into room temperature before use, if  there is salt crystals, shake to make it dissovled, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃ for about 1 week.

6. Notes

1) Return all reagents into room temperature before use, shake it evenly before use, and store back to 2-8℃ after usage. 

2) Do not mix use reagents from different kits and different lot no., prevent the reagents been polluted when using.

3) Substrate and stop solution may have irritation to skin and eyes, be careful to use.

4) Do not expose Substrate to strong light and avoid contact with the oxidant.

5) Brucellosis-Ag coated plates should be sealed and moisture-proof. Put back unused MicroWell plate into dry foil bag and sealed at 2~8 .

6) All wastes should be treated well to avoid pollution before discarding.

7) Strict compliance with the operating instructions can get the best results. Pipetting operation, timing, and washing of the whole process must be precise.

8) Brucellosis-Ag coated plates is disposable, do not repeat use.

7. Test procedure

1) For every test, set 1 well for positive control and 2 wells for negative control;

2) Add sample diluent into each well, 40ul/well;

3) Add serum, negative control and positive control to its corresponding wells, 10ul/well. (Do not mix use the tips);

4) Adding Enzyme Conjugate,50ul/well, shake gentle to mix it evenly, cover it with Adhesive Foil, incubate at 37 ℃ for 60 minutes;

5) Open the adhesive foil, discard the liquid of the well, add diluted washing buffer to each well, 250ul/well, discard the liquid, repeat the above step for 4-6 times, at last flap to dry with the absorbent paper;

6) Add substrate, 100ul/well, mix it evenly then cover it with Adhesive Foil, incubate at 37 ℃ in dark for 15 minutes;

7) Add stop solution, 50ul/well to stop the reaction, measure the result in 10 minutes.

8. Results judgement

Set zero at blank control well, read the OD value at 450nm (630nm as reference).

For the assay to be valid:

OD value of negative control(N) > 0.5, meanwhile positive value (P) blocking rate > 80%

Calculate method:

PI(blocking rate)= {1- (Sample OD value/ Negative control OD average value)}x 100%

Results interpretation

PI(blocking rate)> 70%: Positive

PI(blocking rate)≤ 70%: Negative

9. Storage and expire date

    Store at 2~8in dark, no frozen, expiry date: 12 months.


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pig and dog etc. 

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ISO9001&ISO14001 Certificate 



Company Information

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.


Our advantages at the Diagnostic kits/ ELISA test kits field are as following:


* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.





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