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Quick Details

  • Type: Pathological Analysis Equipments
  • Brand Name: Green Spring
  • Place of Origin: Guangdong, China (Mainland)
  • Model Number: LSY-30035
  • Sample perfomance: Serum
  • Certificate: ISO9001&ISO14001&OHSMS8001
  • MOQ: 1 kit
  • Specifications: 96*2 wells/kit or 96 wells/kit
  • Shelf life: 12 months

Packaging & Delivery

Packaging Details By foam box with ice to maintain cool storage
Packing kit size: 16.5*11.5*12cm
96*2 wells/kit
Delivery Time In 3 days after payment

 Peste des petits ruminants virus (PPRV) antibody ELISA test kit 

Catalog No. LSY-30035

 

Product Description

 

  1. Principle 

This kit is used to detect specific antibody against Peste des petits ruminants virus (PPRV) Antibody in serum qualitatively, for monitoring antibody after PPRV immune and serological diagnosis of infection in sheep

This kit use indirect ELISA method, pured Ovine PPRV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is PPR virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-PPR virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.

 

2. Reagents and contents

Code

Item

Spec.

Code

Item 

Spec.

1

Antigen Coated plates

96 wells

1/2 plate

7

  Stop solution

6/11 ml 

2

Enzyme Conjugate

6/11 ml

8

Positive control

0.8/1.6 ml

3

10X Washing buffer  

50 ml 

9

Negative control

0.8/1.6 ml

4

Substrate A

6/11 ml

10

Serum dilute plate

1/2 plate

5

Substrate B

6/11 ml

11

Adhesive Foil

2/4 pieces

6

Sample diluent

50 ml 

12

Instruction

1 piece

 

3. Material required not provided

1) Micropipette: 0.5-10ul, 10ul-100ul, 100ul-1000ul.

2) Disposable pipette tips.

3) Graduate: 500ml.

4) Microplate Reader: 96 wells with 450/630nm wavelenth.

5) Distilled water or deionized water.

6) Microplate Washer

 

4. Sample preparation

Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis

 

5. Washing buffer preparation

Return 10X Concentrated washing buffer into room temperature before use, is there is salt crystals, shake to make it dissovled, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃ for about 1 week. 

 

6. Sample dilution

Dilute serum at 1:100 on Serum dilute plate(for example: 495ul sample diluent + 5ul serum).

Note: both positive control and negative control do not need dilute, change tips after taking every sample, mark the sample location on plate accurately, mix every sample evenly before adding to the pre-coated micro-wells. 

 

 

 

Our ELISA test kits for Bovine and Sheep 

Product CodeProduct NameSpec
LSY-30031

Foot and mouth disease virus (FMDV) Type O 

Antibody ELISA Test Kit for  bovine and ovine

96*2 well/kit
LSY-30033Foot and mouth disease virus (FMDV) Type Asia-Ⅰ antibody ELISA test kit for bovine and ovine96*2 well/kit
LSY-30034

Foot and mouth disease virus (FMDV) Type A 

antibody ELISA test kit for bovine and ovine

96*2 well/kit
LSY-30040

FMDV NSP Antibody 3ABC ELISA test kit for 

cattle, sheep, goat and pig 

96*2 well/kit
LSY-30035

Peste des petits ruminants virus (PPRV) antibody 

ELISA test kit for ovine

96 well/kit
LSY-30036Brucellosis antibody ELISA kit for bovine, sheep. pig and dog etc. 96 well/kit
LSY-30038Toxoplasma gondii antibody ELISA kit for bovine, sheep96 well/kit

 

 

 

 

 

ISO9001&ISO14001 Certificate 

 

 

 

Company Information

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

  

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

  

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.

 

 

 

 

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