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Peste des petits ruminants virus (PPRV) antibody ELISA test kit
Catalog No. LSY-30035
This kit is used to detect specific antibody against Peste des petits ruminants virus (PPRV) Antibody in serum qualitatively, for monitoring antibody after PPRV immune and serological diagnosis of infection in sheep.
This kit use indirect ELISA method, pured Ovine PPRV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is PPR virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-PPR virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
4) Microplate Reader: 96 wells with 450/630nm wavelenth.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis
5. Washing buffer preparation
Return10X Concentrated washing buffer into room temperature before use, is there is salt crystals, shake to make it dissovled, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃ for about 1 week.
6. Sample dilution
Dilute serum at 1:100 on Serum dilute plate(for example: 495ul sample diluent + 5ul serum).
Note: both positive control and negative control do not need dilute, change tips after taking every sample, mark the sample location on plate accurately, mix every sample evenly before adding to the pre-coated micro-wells.
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We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.
Our advantages at the Diagnostic kits/ ELISA test kits field are as following:
* ISO9001:2008 certificated company
* Own a high-quality R&D team with many years of experience on bio-diagnostic field.
* Professional manufacturer of ELISA test kits, offer OEM service.
*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.
*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.