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Quick Details

  • Type: Pathological Analysis Equipments
  • Brand Name: Green Spring
  • Place of Origin: Guangdong, China (Mainland)
  • Model Number: LSY-30012-2
  • Kit Specs PLATE: 1 x 96 well (Removable 8 wells x 12 strips)
  • Sample Performance: Serum of dog and cat
  • Certificate: ISO9001&ISO14001&OHSMS18001
  • MOQ: 1 kit
  • Incubation time: 60min-30min-15min
  • Shelf life: 12 months

Packaging & Delivery

Packaging Details By foam box with ice to maintain cool storage
96 wells/kit
Delivery Time In 3 days after payment

Rabies virus antibody ELISA kit for Dogs and Cats

Catalog No. LSY-30012-2

Product Description

 

1. Introduction 

The Rabies Virus (RBV) antibody ELISA kit is used to test rabies virus antibody in serum of dogs, cats etc., used to assess the status of rabies vaccination.

This kit use block ELISA method, rabies antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is rabies virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-rabies virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.

 

2. Reagents and contents

Code

Item

Spec.

Code

Item

Spec.

1

RBV-Ag Coated plates

96 wells

6

Stop solution

11 ml

2

Enzyme Conjugate

11 ml

7

Negative  control

1 ml

3

10X Concentrated washing buffer  

100 ml

8

Positive control

0.5 ml

4

Substrate  

11 ml

9

Adhesive Foil

2 pieces

5

Sample diluent

50 ml

10

Instruction sheet

1 piece

 

3. Material required not provided

1) Micropipette: 10ul-100ul, 100ul-1000ul.

2) Disposable pipette tips.

3) Graduate: 500ml.

4) Microplate Reader: 96 wells.

5) Distilled water or deionized water.

6) Microplate Washer

 

4. Sample preparation

Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis

 

5. Washing buffer preparation

Return 10X Concentrated washing buffer into room temperature before use, if  there is salt crystals, shake to make it dissovled, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃ for about 1 week.

 

6. Notes

1) Return all reagents into room temperature before use, shake it evenly before use, and store back to 2-8℃ after usage.

2) Do not mix use reagents from different kits and different lot no., prevent the reagents been polluted when using.

3) Substrate A and stop solution may have irritation to skin and eyes, be careful to use.

4) Do not expose Substrate to strong light and avoid contact with the oxidant.

5) RBV-Ag coated plates should be sealed and moisture-proof. Put back unused MicroWell plate into dry foil bag and sealed at 2~8 ℃.

6) All wastes should be treated well to avoid pullution before discarding.

7) Strict compliance with the operating instructions can get the best results. Pipetting operation, timing, and washing of the whole process must be precise.

8) RBV-Ag coated plates is disposable, do not repeat use.

 

7. Test procedure

1) For every test, set 1 well for positive control and 2 wells for negative control, negative control and positive control do not need to dilute, directly add to its corresponding wells, 100ul/well;

2) Add sample diluent into reaction wells, 90ul/well, then add serum, 10ul/well, blow and beat it to even. (Do not mix use the tips);

3) Cover it with Adhesive Foil, incubate at 37 ℃ for 60 minutes;

4) Open the adhesive foil, discard the liquid of the well, add diluted washing buffer to each well, 250ul/well, discard the liquid, repeat the above step for 4-6 times, at last flap to dry with the absorbent paper;

5) Adding Enzyme Conjugate,100ul/well, cover it with Adhesive Foil, incubate at 37 ℃ for 30 minutes;

6) Open the adhesive foil, discard the liquid of the well, washing for 4-6 times as step 4), remember at last flap to dry with the absorbent paper;

7) Add substrate, 100ul/well, mix it evenly then cover it with Adhesive Foil, incubate at 37 ℃ in dark for 15 minutes;

8) Add stop solution, 50ul/well to stop the reaction, measure the result in 10 minutes.

 

 

 

Our ELISA Kits for Dog 

 

Product CodeProduct NameSpec
LSY-30012Canine rabies virus antibody ELISA kit96 well/kit
LSY-30032Toxoplasma gondii IgG antibody ELISA kit 
for Pet
96 well/kit

 

 

 

ISO9001&ISO14001 Certificate

 

 

 

Company Information

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

 

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

 

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.

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