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Shenzhen Lvshiyuan Biotechnology Co., Ltd.

China (Mainland) | Contact Details
Experience:
Established 2003
6 years OEM
Performance:
Quick Response Rate
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Quick Details

  • Place of Origin: Guangdong, China (Mainland)
  • Brand Name: Green Spring
  • Model Number: LSY-30018
  • Properties: animal disease monitor
  • Specifications: 96 wells/kit
  • Certificate: ISO9001:2008
  • MOQ: 1 kit

Packaging & Delivery

Packaging Details: By foam box with ice to maintain cool storage
Delivery Detail: Deliver goods in 1-3 days after payment

Specifications

Bird Flu(H9N1) antibody ELISA test kit
1.Sensitivity:qualitative analysis
2.Spec:96 wells/kit
3.Sample:serum
4.ISO9001:2008

Bird Flu(H9N1) antibody ELISA test kit 

1.Introduction:This kit use AIV solid phase antigen to detect specific antibody against AIV antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and can be used dividually in multiple times .

Reagents  and contentsCoated microtiter strips concentrated Conjugate solution: Washing solution: Substrate A solutionSubstrate Bsolution: Sample dilution:Stopping solution  Positive contro Negative control:Instruction sheet 
 quantity 8 1 1 1 1 1 1 1 1 1
 condition ready to use ready to use ready to use ready to use ready to use ready to use ready to use ready to use ready to use 

2. ELISA procedures procedures

1. Diluting concentrated washing solution
Dilute the concentrated washing solution with distilled water or deionized water at 1:10, e.g. add 270 ml distilled water in 30 ml concentrated washing solution, and mix thoroughly.
2. Sample preparation:
Dilute the serum sample with sample dilution at 1:100, e.g. 5 μ l serum + 495 μ l sample dilution,mix thoroughly.
3. Adding samples and controls
Leave well A1 as reagent blank. Pipette controls and samples as follows:
100 μ l of negative control and positive control respectively, and 100 μ l diluted samples each into remaining wells.Incubation at 37 °C for 30 minutes. Discard liquid of the wells and fill all wells with diluted washing solution, incubate for 1 minute and discard. Repeat washing procedure two more times as above.At the end of the washing step, carefully remove remaining fluid by tapping the strips on th e tissue paper prior to the next step.

4. Adding conjugate solution
Add 1 drop conjugate solution into all wells and incubate at 37 °C for 30 minutes. Discard liquid of the wells and wash 3 times as described in step 3.
5. Adding substrates
Add one drop of substrate A solution and B solution respectively, incubate at 37 °C for 10 minutes.
Add one drop of stopping solution into all wells. Before reading results with ELISA microtiter plate reader, set the reagent blank well A1 to zero . Measure the absorbance of all wells at 450 nm(reference wavelength: 620 nm).

 

ISO9001:2008 Certificate

 

 

Company Information

  

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

 

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

 

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.

 

We have other bird disease diagnostic kits

LSY-30011Bird Flu/Avian Influenza Virus antibody ELISA test kit96 wells/kitqualitativeSerum
LSY-30012Canine rabies virus antibody ELISA kit96 wells/kitqualitativeSerum
LSY-30013Newcastle disease virus antibody ELISA kit96 wells/kitqualitativeSerum
LSY-30014Infectious Bursal disease virus(IBDV) antibody ELISA kit96 wells/kitqualitativeSerum
LSY-30015Avian EDS76 antibody ELISA kit 96 wells/kitqualitativeSerum
LSY-30016Avian Marek's disease virus antibody ELISA kit    96 wells/kitqualitativeSerum
LSY-30017Avian Infectious bursal disease(IBD) antibody ELISA test kit96 wells/kitqualitativeSerum
LSY-30018Bird Flu(H9N1) antibody ELISA test kit96 wells/kitqualitativeSerum

 

 

High-tech Enterprise Certificate

 

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