- Place of Origin: Guangdong, China (Mainland)
- Brand Name: Green Spring
- Model Number: LSY-30018
- Properties: animal disease monitor
- Specifications: 96 wells/kit
- Certificate: ISO9001:2008
- MOQ: 1 kit
Packaging & Delivery
|Packaging Details:||By foam box with ice to maintain cool storage|
|Delivery Detail:||Deliver goods in 1-3 days after payment|
SpecificationsBird Flu(H9N1) antibody ELISA test kit
Bird Flu(H9N1) antibody ELISA test kit
1.Introduction:This kit use AIV solid phase antigen to detect specific antibody against AIV antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and can be used dividually in multiple times .
|Reagents and contents||Coated microtiter strips||concentrated Conjugate solution:||Washing solution:||Substrate A solution||Substrate Bsolution:||Sample dilution:||Stopping solution||Positive contro||Negative control:||Instruction sheet|
|condition||ready to use||ready to use||ready to use||ready to use||ready to use||ready to use||ready to use||ready to use||ready to use|
2. ELISA procedures procedures
1. Diluting concentrated washing solution
Dilute the concentrated washing solution with distilled water or deionized water at 1:10, e.g. add 270 ml distilled water in 30 ml concentrated washing solution, and mix thoroughly.
2. Sample preparation:
Dilute the serum sample with sample dilution at 1:100, e.g. 5 μ l serum + 495 μ l sample dilution,mix thoroughly.
3. Adding samples and controls
Leave well A1 as reagent blank. Pipette controls and samples as follows:
100 μ l of negative control and positive control respectively, and 100 μ l diluted samples each into remaining wells.Incubation at 37 °C for 30 minutes. Discard liquid of the wells and fill all wells with diluted washing solution, incubate for 1 minute and discard. Repeat washing procedure two more times as above.At the end of the washing step, carefully remove remaining fluid by tapping the strips on th e tissue paper prior to the next step.
4. Adding conjugate solution
Add 1 drop conjugate solution into all wells and incubate at 37 °C for 30 minutes. Discard liquid of the wells and wash 3 times as described in step 3.
5. Adding substrates
Add one drop of substrate A solution and B solution respectively, incubate at 37 °C for 10 minutes.Add one drop of stopping solution into all wells. Before reading results with ELISA microtiter plate reader, set the reagent blank well A1 to zero . Measure the absorbance of all wells at 450 nm(reference wavelength: 620 nm).
We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.
Our advantages at the Diagnostic kits/ ELISA test kits field are as following:
* ISO9001:2008 certificated company
* Own a high-quality R&D team with many years of experience on bio-diagnostic field.
* Professional manufacturer of ELISA test kits, offer OEM service.
*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.
*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.
We have other bird disease diagnostic kits
|LSY-30011||Bird Flu/Avian Influenza Virus antibody ELISA test kit||96 wells/kit||qualitative||Serum|
|LSY-30012||Canine rabies virus antibody ELISA kit||96 wells/kit||qualitative||Serum|
|LSY-30013||Newcastle disease virus antibody ELISA kit||96 wells/kit||qualitative||Serum|
|LSY-30014||Infectious Bursal disease virus(IBDV) antibody ELISA kit||96 wells/kit||qualitative||Serum|
|LSY-30015||Avian EDS76 antibody ELISA kit||96 wells/kit||qualitative||Serum|
|LSY-30016||Avian Marek's disease virus antibody ELISA kit||96 wells/kit||qualitative||Serum|
|LSY-30017||Avian Infectious bursal disease(IBD) antibody ELISA test kit||96 wells/kit||qualitative||Serum|
|LSY-30018||Bird Flu(H9N1) antibody ELISA test kit||96 wells/kit||qualitative||Serum|
High-tech Enterprise Certificate
|Buyer||Product Name||Price||Quantity||Order Time(GMT+8)|